Primers needed to clone into pET-27b(+) vector. Cut sites will be EcoRI and HindIII
Forward Primer:
ATCTATACTTGAATTCGATGGCGATTTCTCTGCT
Reverse Primer (Reverse Complemented):
GTAACCGGCTAAAAGCTTTCAATTTTGGTAAGA
Notes: 1. Temp down 2. Truncate 3. Clone into a pET vector
Adding detergents, high salt concentrations, etc. didn't necessarily work when added to normal lysis buffer in preventing aggregation of FabI protein. It should be noted that the protein actually became soluble with these added conditions more than normal conditions.
Protein ID (NP or XP #) or Wolbachia#:Protein Accession # : AAW71058.1 Wolbachia # : Q5GSG6 Locus:YP_198300
MHS Rank: 114 GCS Rank: 137 on WOLTR spreadsheet
Organism: Wolbachia endosymbiont strain TRS of Brugia malayi
Etiologic Risk Group (see link below): N/A
Molecular Weight:
29360.4 kDa
Background/Disease Information:
Wolbachia are gram-negative bacteria that form intracellular inherited infections in many invertebrates. They are extremely common with 20-75% of all insects being infected. Moreover they infect numerous non-insect invertebrates including nematodes, mites and spiders. The limits of the host range of Wolbachia are not fully appreciated at this time. Much of the success of Wolbachia can be attributed to the diverse phenotypes that result from infection. These range from classical mutualism to reproductive parasitism as characterized by the ability of Wolbachia to override chromosomal sex determination, induce parthenogenesis, selectively kill males, influence sperm competition and generate cytoplasmic incompatibility in early embryos.
Reaction catalyzed by enoyl-[acyl-carrier-protein] reductase (NADH) (1.3.1.9)
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Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): -- link to Sigma (or other company) page for assay or assay reagents (substrates): see below -- link to paper that contains assay information:see below; full link-------http://pubs.acs.org/doi/full/10.1021/jm020050%2B
Structure Available (PDB or Homology model): http://www.rcsb.org/pdb/explore/explore.do?structureId=3k2e -- PDB # or closest PDB entry if using homology model: 3K2E -- Query Coverage (if not direct match): 97% -- Max Ident (if not direct match): 65%
Druggable Target (see Databases for this): Found to be druggable in E. Coli
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol or etc): Amino Acid Sequence:
length of your protein in Amino Acids: 270 Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website Molar Extinction coefficient of your protein at 280 nm wavelength: CDS Gene Sequence:
07/29/13
Primers needed to clone into pET-27b(+) vector. Cut sites will be EcoRI and HindIII
Forward Primer:
ATCTATACTTGAATTCGATGGCGATTTCTCTGCT
Reverse Primer (Reverse Complemented):
GTAACCGGCTAAAAGCTTTCAATTTTGGTAAGA
Notes: 1. Temp down 2. Truncate 3. Clone into a pET vector
Adding detergents, high salt concentrations, etc. didn't necessarily work when added to normal lysis buffer in preventing aggregation of FabI protein. It should be noted that the protein actually became soluble with these added conditions more than normal conditions.
http://www.embl.de/pepcore/pepcore_services/protein_expression/ecoli/improving_protein_solubility/index.html
102712
GST tag info:
http://cshprotocols.cshlp.org/content/2007/4/pdb.prot4738.full
GST with His6 tag. LIC cloning (similar but different to our LIC cloning)
http://www.addgene.org/29655/
http://qb3.berkeley.edu/qb3/macrolab/projects.cfm
From SGC - but requires special cloning system...
http://www.addgene.org/26101/
http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php
NCBI Gene # or RefSeq#: 3266932
Protein ID (NP or XP #) or Wolbachia#: Protein Accession # : AAW71058.1 Wolbachia # : Q5GSG6 Locus:YP_198300
MHS Rank: 114 GCS Rank: 137 on WOLTR spreadsheet
Organism: Wolbachia endosymbiont strain TRS of Brugia malayi
Etiologic Risk Group (see link below): N/A
Molecular Weight:
29360.4 kDa
Background/Disease Information:
Wolbachia are gram-negative bacteria that form intracellular inherited infections in many invertebrates. They are extremely common with 20-75% of all insects being infected. Moreover they infect numerous non-insect invertebrates including nematodes, mites and spiders. The limits of the host range of Wolbachia are not fully appreciated at this time. Much of the success of Wolbachia can be attributed to the diverse phenotypes that result from infection. These range from classical mutualism to reproductive parasitism as characterized by the ability of Wolbachia to override chromosomal sex determination, induce parthenogenesis, selectively kill males, influence sperm competition and generate cytoplasmic incompatibility in early embryos.
from: https://www.wolbachiawebsite.org/about.cfm
Essentiality of this protein: Found to be essential in E. Coli lipid synthesis
http://tdrtargets.org/targets/view?gene_id=232720
Complex of enzymes: No
EC#: 1.3.1.9
Link to BRENDA EC# page: http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay or assay reagents (substrates): see below
-- link to paper that contains assay information:see below; full link-------http://pubs.acs.org/doi/full/10.1021/jm020050%2B
Structure Available (PDB or Homology model):
http://www.rcsb.org/pdb/explore/explore.do?structureId=3k2e
-- PDB # or closest PDB entry if using homology model: 3K2E
-- Query Coverage (if not direct match): 97%
-- Max Ident (if not direct match): 65%
Druggable Target (see Databases for this): Found to be druggable in E. Coli
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol or etc):
Amino Acid Sequence:
1 <span class="ff_line">maisllqgkk glitgiinkr siaygivktl sehgaesavt yqneivkerl lsiaaelnve</span> 61 <span class="ff_line">lvlncdvane gtiddvfksi eekwgtldfl vhaiafsdkn elsgkyvnts lnnfqnamni</span> 121 <span class="ff_line">scysftalaq raekmmpngg slltlsyyga ekvmpnynvm glckaaleas vkylacdlgp</span> 181 <span class="ff_line">qnirvnaisa gpirtlassg isdfhfisew nrnnsplrrn ttledvgkaa lyllsdlssg</span> 241 <span class="ff_line">ttgeilhvds gynvvgmkai dsniinsyqn</span>length of your protein in Amino Acids: 270
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
Molar Extinction coefficient of your protein at 280 nm wavelength:
CDS Gene Sequence:
ATGGCGATTTCTCTGCTGCAGGGCAAAAAAGGTCTGATCACCGGCATTATCAACAAACGT
TCTATCGCGTACGGTATCGTTAAAACCCTGAGCGAACATGGTGCAGAATCTGCGGTTACC
TACCAGAACGAAATCGTCAAAGAACGTCTGCTGAGCATTGCCGCGGAACTGAACGTTGAA
CTGGTTCTGAACTGCGACGTTGCGAACGAAGGTACCATCGACGACGTTTTCAAATCTATC
GAAGAAAAATGGGGTACGCTCGACTTTCTGGTTCACGCAATCGCATTCTCTGACAAAAAC
GAACTGTCTGGTAAATACGTTAACACCTCTCTGAACAATTTCCAAAACGCCATGAACATC
TCTTGCTACTCTTTCACTGCGCTGGCGCAACGCGCTGAAAAGATGATGCCAAATGGTGGT
TCTCTCCTGACGCTGTCTTACTACGGTGCGGAAAAAGTTATGCCGAACTACAATGTTATG
GGTCTGTGCAAAGCGGCTCTGGAAGCGTCTGTTAAATACCTGGCGTGCGACCTGGGTCCG
CAGAACATCCGTGTAAACGCGATCTCTGCGGGTCCGATCCGTACCCTGGCGAGCTCTGGT
ATTTCTGACTTCCACTTCATCAGCGAATGGAACCGTAACAACTCTCCGCTGCGTCGTAAC
ACCACCCTGGAAGACGTTGGTAAAGCCGCCCTGTACCTGCTGTCTGACCTGTCCTCTGGC
ACGACGGGTGAGATCCTGCACGTTGACTCTGGTTACAACGTAGTTGGTATGAAAGCGATC
GATTCTAACATCATTAATTCTTACCAAAATTGA
GC% Content for gene: 49.2%
CDS Gene Sequence (codon optimized) - after having done Primer Design:
ATGGCGATTTCTCTGCTGCAGGGCAAAAAAGGTCTGATCACCGGCATTATCAACAAACGT
TCTATCGCGTACGGTATCGTTAAAACCCTGAGCGAACATGGTGCAGAATCTGCGGTTACC
TACCAGAACGAAATCGTCAAAGAACGTCTGCTGAGCATTGCCGCGGAACTGAACGTTGAA
CTGGTTCTGAACTGCGACGTTGCGAACGAAGGTACCATCGACGACGTTTTCAAATCTATC
GAAGAAAAATGGGGTACGCTCGACTTTCTGGTTCACGCAATCGCATTCTCTGACAAAAAC
GAACTGTCTGGTAAATACGTTAACACCTCTCTGAACAATTTCCAAAACGCCATGAACATC
TCTTGCTACTCTTTCACTGCGCTGGCGCAACGCGCTGAAAAGATGATGCCAAATGGTGGT
TCTCTCCTGACGCTGTCTTACTACGGTGCGGAAAAAGTTATGCCGAACTACAATGTTATG
GGTCTGTGCAAAGCGGCTCTGGAAGCGTCTGTTAAATACCTGGCGTGCGACCTGGGTCCG
CAGAACATCCGTGTAAACGCGATCTCTGCGGGTCCGATCCGTACCCTGGCGAGCTCTGGT
ATTTCTGACTTCCACTTCATCAGCGAATGGAACCGTAACAACTCTCCGCTGCGTCGTAAC
ACCACCCTGGAAGACGTTGGTAAAGCCGCCCTGTACCTGCTGTCTGACCTGTCCTCTGGC
ACGACGGGTGAGATCCTGCACGTTGACTCTGGTTACAACGTAGTTGGTATGAAAGCGATC
GATTCTAACATCATTAATTCTTACCAAAATTGA
GC% Content for gene (codon optimized): 49.2%
Amino Acid Sequence:
MAISLLQGKKGLITGIINKRSIAYGIVKTLSEHGAESAVTYQNEIVKERLLSIAAELNVE LVLNCDVANEGTIDDVFKSIEEKWGTLDFLVHAIAFSDKNELSGKYVNTSLNNFQNAMNI SCYSFTALAQRAEKMMPNGGSLLTLSYYGAEKVMPNYNVMGLCKAALEASVKYLACDLGP QNIRVNAISAGPIRTLASSGISDFHFISEWNRNNSPLRRNTTLEDVGKAALYLLSDLSSG TTGEILHVDSGYNVVGMKAIDSNIINSYQN
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):
Upstream: TACTTCCAATCCATGGCGATTTCTCTGCTG
Downstream: TATCCACCTTTACTGTCAATTTTGGTAAGAATTAATG
FabI used in fatty acid/ lipid biosynthesis
http://www.uniprot.org/uniprot/Q2GKM8
Journal Article:
http://www.ncbi.nlm.nih.gov/pubmed/12109908
Substrates needed: From Sigma
0.25 mM crotonoyl-CoA: Product Number: 28007, 5 mg $246.00
50 μM NADH: Product Number: N4505, 100 mg $58.80
http://www.ncbi.nlm.nih.gov/protein/58419043
Q5GSG6
Protein Accession # AAW71058.1
Primer Link LogFile from DNA works Primer Design: https://docs.google.com/open?id=0B4O2KqKh2q_-RGlfc3YyVnJ6ZFU
Link to Primer Design -
https://docs.google.com/file/d/0B4O2KqKh2q_-eFNGQUhuc05kRDA/edit
Plasmid (FABI) inserted into vector
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTT
TGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGT
AGATCTGGGTACCGAGAACCTGTACTTCCAATCCATGGCGATTTCTCTGCTGCAGGGCAA
AAAAGGTCTGATCACCGGCATTATCAACAAACGTTCTATCGCGTACGGTATCGTTAAAAC
CCTGAGCGAACATGGTGCAGAATCTGCGGTTACCTACCAGAACGAAATCGTCAAAGAACG
TCTGCTGAGCATTGCCGCGGAACTGAACGTTGAACTGGTTCTGAACTGCGACGTTGCGAA
CGAAGGTACCATCGACGACGTTTTCAAATCTATCGAAGAAAAATGGGGTACGCTCGACTT
TCTGGTTCACGCAATCGCATTCTCTGACAAAAACGAACTGTCTGGTAAATACGTTAACAC
CTCTCTGAACAATTTCCAAAACGCCATGAACATCTCTTGCTACTCTTTCACTGCGCTGGC
GCAACGCGCTGAAAAGATGATGCCAAATGGTGGTTCTCTCCTGACGCTGTCTTACTACGG
TGCGGAAAAAGTTATGCCGAACTACAATGTTATGGGTCTGTGCAAAGCGGCTCTGGAAGC
GTCTGTTAAATACCTGGCGTGCGACCTGGGTCCGCAGAACATCCGTGTAAACGCGATCTC
TGCGGGTCCGATCCGTACCCTGGCGAGCTCTGGTATTTCTGACTTCCACTTCATCAGCGA
ATGGAACCGTAACAACTCTCCGCTGCGTCGTAACACCACCCTGGAAGACGTTGGTAAAGC
CGCCCTGTACCTGCTGTCTGACCTGTCCTCTGGCACGACGGGTGAGATCCTGCACGTTGA
CTCTGGTTACAACGTAGTTGGTATGAAAGCGATCGATTCTAACATCATTAATTCTTACCA
AAATTGACAGTAAAGGTGGATACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCC
GCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA
GCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAA
CGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACG
CGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTA
CACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGT
TCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTG
CTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCAT
CGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGAC
TCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAG
GGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACG
CGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGC
GCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATT
AATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTA
TCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAG
TTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATA
CAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTG
ACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACA
GGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGT
GATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGA
ATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCA
GGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCAT
GCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGC
CAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTC
AGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGC
CCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAAT
CGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTG
TTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTT
CCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCT
GCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCC
GGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACC
AAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC
GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTC
GTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTG
AACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATA
CCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA
TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGC
CTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTG
ATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTT
CCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGT
GGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGA
GCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTAC
GCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGAT
GCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGC
CCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCG
CTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCAT
CACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCAC
AGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCT
GGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCT
CCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGC
TCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAAC
AACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCT
TCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCC
GGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAAC
CGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACG
TTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCC
GGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAA
TGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGG
CGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGC
GGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAA
AGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGA
CTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAAC
TTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGC
TGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAG
ATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATC
TGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTT
TGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGA
TTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAAT
GGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGT
CGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCA
AGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCC
AGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCT
TTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGA
TCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAG
GTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGA
ATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGG
CTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACA
TCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAT
CATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCC
CTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGC
CGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCC
TGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTC
CCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCC
GGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAAT
Homology model site:
http://www1.jcsg.org/scripts/prod/scwrl/serve.cgi
TMPred site:
http://www.ch.embnet.org/software/TMPRED_form.html